Experimental research of the wave front distortions caused by AOTF-based spectral imagers for biomedical applications

A. S. Machikhin; V. I. Batshev; Yu. A. Firsenkova

doi:10.1117/12.2179624

19 March 2015 Experimental research of the wave front distortions caused by AOTF-based spectral imagers for biomedical applications

A. S. Machikhin, V. I. Batshev, Yu. A. Firsenkova

Author Affiliations +

Proceedings Volume 9448, Saratov Fall Meeting 2014: Optical Technologies in Biophysics and Medicine XVI; Laser Physics and Photonics XVI; and Computational Biophysics; 94481C (2015) https://doi.org/10.1117/12.2179624
Event: Saratov Fall Meeting 2014, 2014, Saratov, Russian Federation

Abstract

The acousto-optic-tunable-filter-based (AOTF-based) spectral imaging systems for non-invasive photoluminescence diagnostics are discussed. The problem of image distortion caused by AO interaction which causes the inaccuracy of spectral measurements is considered. The existing methods of AOTF calibration do not provide the full information about the image structure and the individual aberrations. It is proposed to reconstruct the optical path difference (OPD) caused by AOTF. For this purpose the experimental setup based on Shack-Hartmann sensor was assembled. It is shown that it allows defining the magnitude of the main aberrations of double AOTF are meridional and sagittal coma and some high-order components. Spectral dependence of OPD and its components is rather weak and shows a small chromatic image distortion. The obtained data is in good correspondence with the results of the theoretical studies of the double AOTFs. Wave front measurement may be effectively used for the study and optimization of existing AOTF-based spectral imagers. The effectiveness of this technique is demonstrated on the measurement of the spectrums of real biomedical tissues obtained by the endoscopic AOTF-based imager.

Citation Download Citation

A. S. Machikhin, V. I. Batshev, and Yu. A. Firsenkova "Experimental research of the wave front distortions caused by AOTF-based spectral imagers for biomedical applications", Proc. SPIE 9448, Saratov Fall Meeting 2014: Optical Technologies in Biophysics and Medicine XVI; Laser Physics and Photonics XVI; and Computational Biophysics, 94481C (19 March 2015); https://doi.org/10.1117/12.2179624

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