Development of color micro optical-CT: evaluation using phantom and biological samples

C. Murata; A. Teramoto; C. Kaneko; H. Fujita

doi:10.1117/12.2081681

19 March 2015 Development of color micro optical-CT: evaluation using phantom and biological samples

C. Murata, A. Teramoto, C. Kaneko, H. Fujita

Proceedings Volume 9417, Medical Imaging 2015: Biomedical Applications in Molecular, Structural, and Functional Imaging; 941722 (2015) https://doi.org/10.1117/12.2081681
Event: SPIE Medical Imaging, 2015, Orlando, Florida, United States

Abstract

Micro-optical computed tomography (MOCT) is a method for performing image reconstruction using microscopic images to obtain tomographic images of small samples. Compared with conventional observation methods, it offers the possibility to obtain tomograpic images without distortion, and create three-dimensional images. However, MOCT system which developed previously outputs monochrome images, while useful color information could not be obtained from the analysis of the sample. Therefore, we focused on the features that simplify the wavelength measurement of visible light, and developed a color MOCT system that can obtain color tomographic images. In this study, we acquired tomographic images of phantom and biological samples, and evaluated its usefulness. In this system, a digital single-lens reflex camera was used as a detector that was connected to a stereoscopic microscope, and projection images were obtained by rotating the sample. The sample was fixed in the test tube by carrageenan. The projection images were obtained from various projection angles followed by decomposing the R, G and B components. Subsequently, we performed image reconstruction for each component using filtered back projection. Finally, color tomographic image was obtained by combining the three-color component images. In the experiments, we scanned a color phantom and biological samples and evaluated the color and shape reproducibility. As a result, it was found that the color and shape of the tomographic images were similar to those of the samples. These results indicate that the proposed system may be useful to obtain the three-dimensional color structure of biological samples.

Citation Download Citation

C. Murata, A. Teramoto, C. Kaneko, and H. Fujita "Development of color micro optical-CT: evaluation using phantom and biological samples", Proc. SPIE 9417, Medical Imaging 2015: Biomedical Applications in Molecular, Structural, and Functional Imaging, 941722 (19 March 2015); https://doi.org/10.1117/12.2081681

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
6 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Tomography

Image restoration

Microscopes

Biological research

Biomedical optics

Cameras

CT reconstruction

Show All Keywords

Keywords/Phrases

Search In:

Publication Years