Real-time characterization of the neuronal response to osmotic shock by digital holographic microscopy

Maria Gomariz; Isabel Garcia; Cristina Soto-Sánchez; Gema Martínez-Navarrete; Eduardo Fernández; Antonio Fimia

doi:10.1117/12.2179116

8 May 2015 Real-time characterization of the neuronal response to osmotic shock by digital holographic microscopy

Maria Gomariz, Isabel Garcia, Cristina Soto-Sánchez, Gema Martínez-Navarrete, Eduardo Fernández, Antonio Fimia

Proceedings Volume 9508, Holography: Advances and Modern Trends IV; 950803 (2015) https://doi.org/10.1117/12.2179116
Event: SPIE Optics + Optoelectronics, 2015, Prague, Czech Republic

Abstract

Digital holographic microscopy has numerous applications in biology for visualizing living cells and 3D tissues. This technique allows for the direct visualization of biological structures avoiding invasive and phototoxic procedures such as fixation and dying processing. In this study we have characterized the morphometry changes of neurons subject to osmolarity changes. For this purpose, we have measured the variations of the amplitude and the oscillation frequency of the plasmatic membrane, as well as the volume changes of the cells before the osmotic shock. There was a relation between the neural culture ageing and its behavioral changes. "Long-term" cultures that had not previously been studied were used to analyze the behavioral changes in aged cells.

Citation Download Citation

Maria Gomariz, Isabel Garcia, Cristina Soto-Sánchez, Gema Martínez-Navarrete, Eduardo Fernández, and Antonio Fimia "Real-time characterization of the neuronal response to osmotic shock by digital holographic microscopy", Proc. SPIE 9508, Holography: Advances and Modern Trends IV, 950803 (8 May 2015); https://doi.org/10.1117/12.2179116

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