Lab-on-a-chip enabled HLA diagnostic: combined sample preparation and real time PCR for HLA-B57 diagnosis

Claudia Gärtner; Holger Becker; Nadine Hlawatsch; Richard Klemm; Christian Moche; Sebastian Schattschneider; Rainer Frank; Andreas Willems

doi:10.1117/12.2178897

13 May 2015 Lab-on-a-chip enabled HLA diagnostic: combined sample preparation and real time PCR for HLA-B57 diagnosis

Claudia Gärtner, Holger Becker, Nadine Hlawatsch, Richard Klemm, Christian Moche, Sebastian Schattschneider, Rainer Frank, Andreas Willems

Author Affiliations +

Proceedings Volume 9490, Advances in Global Health through Sensing Technologies 2015; 94900F (2015) https://doi.org/10.1117/12.2178897
Event: SPIE Sensing Technology + Applications, 2015, Baltimore, MD, United States

Abstract

The diverse human HLA (human leukocyte antigen) system is responsible for antigen presentation and recognition. It is essential for the immune system to maintain a stable defense line, but also is also involved in autoimmunity as well as metabolic disease. HLA-haplotype (HLA-B27), for instance, is associated with inflammatory diseases such as Bechterew's disease. The administration of the HIV drug Abacavir in combination with another HLA-haplotype (HLAB57) is associated with severe hypersensitivity reactions. Accordingly, the HLA status has to be monitored for diagnosis or prior to start of therapy. Along this line, a miniaturized microfluidic platform has been developed allowing performing the complete analytical process from “sample-in” to “answer-out” in a point-of-care environment. The main steps of the analytical cascade inside the integrated system are blood cell lysis and DNA isolation, DNA purification, real-time PCR and quantitative monitoring of the rise of a fluorescent signal appearing during the PCR based sequence amplification. All bio-analytical steps were intended to be performed inside one chip and will be actuated, controlled and monitored by a matching device. This report will show that all required processes are established and tested and all device components work well and interact with the functional modules on the chips in a harmonized fashion.

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Claudia Gärtner, Holger Becker, Nadine Hlawatsch, Richard Klemm, Christian Moche, Sebastian Schattschneider, Rainer Frank, and Andreas Willems "Lab-on-a-chip enabled HLA diagnostic: combined sample preparation and real time PCR for HLA-B57 diagnosis", Proc. SPIE 9490, Advances in Global Health through Sensing Technologies 2015, 94900F (13 May 2015); https://doi.org/10.1117/12.2178897

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KEYWORDS

Blood

Microfluidics

Lab on a chip

Magnetism

Diagnostics

Point-of-care devices

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