A homogeneous biochemiluminescent assay for detection of influenza

Kwok Min Hui; Xiao Jing Li; Lu Pan; X. James Li

doi:10.1117/12.2183395

13 May 2015 A homogeneous biochemiluminescent assay for detection of influenza

Kwok Min Hui, Xiao Jing Li, Lu Pan, X. James Li

Proceedings Volume 9490, Advances in Global Health through Sensing Technologies 2015; 94900N (2015) https://doi.org/10.1117/12.2183395
Event: SPIE Sensing Technology + Applications, 2015, Baltimore, MD, United States

Abstract

Current methods of rapid detection of influenza are based on detection of the nucleic acids or antigens of influenza viruses. Since influenza viruses constantly mutate leading to appearance of new strains or variants of viruses, these detection methods are susceptible to genetic changes in influenza viruses. Type A and B influenza viruses contain neuraminidase, an essential enzyme for virus replication which enables progeny influenza viruses leave the host cells to infect new cells. Here we describe an assay method, the homogeneous biochemiluminescent assay (HBA), for rapid detection of influenza by detecting viral neuraminidase activity. The assay mimics the light production process of a firefly: a viral neuraminidase specific substrate containing a luciferin moiety is cleaved in the presence of influenza virus to release luciferin, which becomes a substrate to firefly luciferase in a light production system. All reagents can be formulated in a single reaction mix so that the assay involves only one manual step, i.e., sample addition. Presence of Type A or B influenza virus in the sample leads to production of strong, stable and easily detectable light signal, which lasts for hours. Thus, this influenza virus assay is suitable for use in point-of-care settings.

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Kwok Min Hui, Xiao Jing Li, Lu Pan, and X. James Li "A homogeneous biochemiluminescent assay for detection of influenza", Proc. SPIE 9490, Advances in Global Health through Sensing Technologies 2015, 94900N (13 May 2015); https://doi.org/10.1117/12.2183395

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KEYWORDS

Viruses

Signal detection

Optical testing

Point-of-care devices

Proteins

Temperature metrology

Genetics

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