Shaping field for deep tissue microscopy

J. Colon; H. Lim

doi:10.1117/12.2176883

22 May 2015 Shaping field for deep tissue microscopy

J. Colon, H. Lim

Proceedings Volume 9495, Three-Dimensional Imaging, Visualization, and Display 2015; 94950G (2015) https://doi.org/10.1117/12.2176883
Event: SPIE Sensing Technology + Applications, 2015, Baltimore, MD, United States

Abstract

Information capacity of a lossless image-forming system is a conserved property determined by two imaging parameters – the resolution and the field of view (FOV). Adaptive optics improves the former by manipulating the phase, or wavefront, in the pupil plane. Here we describe a homologous approach, namely adaptive field microscopy, which aims to enhance the FOV by controlling the phase, or defocus, in the focal plane. In deep tissue imaging, the useful FOV can be severely limited if the region of interest is buried in a thick sample and not perpendicular to the optic axis. One must acquire many z-scans and reconstruct by post-processing, which exposes tissue to excessive radiation and is also time consuming. We demonstrate the effective FOV can be substantially enhanced by dynamic control of the image plane. Specifically, the tilt of the image plane is continuously adjusted in situ to match the oblique orientation of the sample plane within tissue. The utility of adaptive field microscopy is tested for imaging tissue with non-planar morphology. Ocular tissue of small animals was imaged by two-photon excited fluorescence. Our results show that adaptive field microscopy can utilize the full FOV. The freedom to adjust the image plane to account for the geometrical variations of sample could be extremely useful for 3D biological imaging. Furthermore, it could facilitate rapid surveillance of cellular features within deep tissue while avoiding photo damages, making it suitable for in vivo imaging.

Citation Download Citation

J. Colon and H. Lim "Shaping field for deep tissue microscopy", Proc. SPIE 9495, Three-Dimensional Imaging, Visualization, and Display 2015, 94950G (22 May 2015); https://doi.org/10.1117/12.2176883

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
6 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Microscopy

Tissues

Mirrors

Visualization

Deep tissue imaging

3D image processing

Imaging systems

Show All Keywords

Keywords/Phrases

Search In:

Publication Years