Opto-fluidic flow analysis for monitoring of immunity levels

A. Mohan; A, Bharadwaj; N. Marshkole; T. Saiyed; A. Prabhakar

doi:10.1117/12.2182901

15 June 2015 Opto-fluidic flow analysis for monitoring of immunity levels

A. Mohan, A, Bharadwaj, N. Marshkole, T. Saiyed, A. Prabhakar

Proceedings Volume 9654, International Conference on Optics and Photonics 2015; 96540W (2015) https://doi.org/10.1117/12.2182901
Event: International Conference on Optics and Photonics 2015, 2015, Kolkata, India

Abstract

We describes the design, development and testing of a cost effective and miniaturized version of a flow analyzer. It is designed to detect fluorescence labeled immunocytes in human blood sample. Availing of advancements in micro fluidics and nanolithographic technique, we fabricated a PDMS based device with integrated micro channels for accommodating the optical fibers. The lensed fibers serves as the waveguides for illumination and collection of laser and scattered signals respectively. As a cell crosses the interrogation point the forward scatter, side scatter and fluorescence are detected. Photomultiplier tubes used in conventional flow cytometers have been replaced here with APDs (avalanche photo detectors) and supplemented with digital signal processing. The prototype was tested with different sized polymer beads as well as the live cells.

Citation Download Citation

A. Mohan, A, Bharadwaj, N. Marshkole, T. Saiyed, and A. Prabhakar "Opto-fluidic flow analysis for monitoring of immunity levels", Proc. SPIE 9654, International Conference on Optics and Photonics 2015, 96540W (15 June 2015); https://doi.org/10.1117/12.2182901

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