Multi-illumination Gabor holography recorded in a single camera snap-shot for high-resolution phase retrieval in digital in-line holographic microscopy

Martin Sanz; Jose Angel Picazo-Bueno; Javier Garcia; Vicente Micó

doi:10.1117/12.2184792

22 June 2015 Multi-illumination Gabor holography recorded in a single camera snap-shot for high-resolution phase retrieval in digital in-line holographic microscopy

Martin Sanz, Jose Angel Picazo-Bueno, Javier Garcia, Vicente Micó

Author Affiliations +

Proceedings Volume 9529, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials II; 95290B (2015) https://doi.org/10.1117/12.2184792
Event: SPIE Optical Metrology, 2015, Munich, Germany

Abstract

In this contribution we introduce MISHELF microscopy, a new concept and design of a lensless holographic microscope based on wavelength multiplexing, single hologram acquisition and digital image processing. The technique which name comes from Multi-Illumination Single-Holographic-Exposure Lensless Fresnel microscopy, is based on the simultaneous illumination and recording of three diffraction patterns in the Fresnel domain. In combination with a novel and fast iterative phase retrieval algorithm, MISHELF microscopy is capable of high-resolution (micron range) phase-retrieved (twin image elimination) biological imaging of dynamic events (video rate recording speed) since it avoids the time multiplexing needed for the in-line hologram sequence recording when using conventional phase-shifting or phase retrieval algorithms. MISHELF microscopy is validated using two different experimental layouts: one using RGB illumination and detection schemes and another using IRRB as illumination while keeping the RGB color camera as detection device. Preliminary experimental results are provided for both experimental layouts using a synthetic object (USAF resolution test target).

Citation Download Citation

Martin Sanz, Jose Angel Picazo-Bueno, Javier Garcia, and Vicente Micó "Multi-illumination Gabor holography recorded in a single camera snap-shot for high-resolution phase retrieval in digital in-line holographic microscopy", Proc. SPIE 9529, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials II, 95290B (22 June 2015); https://doi.org/10.1117/12.2184792

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KEYWORDS

RGB color model

Digital holography

Image retrieval

Microscopy

Holograms

Phase retrieval

Holography

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