Confocal Raman study of aging process in diabetes mellitus human voluntaries

Liliane Pereira; Claudio Alberto Téllez Soto; Laurita dos Santos; Syed Mohammed Ali; Priscila Pereira Fávero; Airton A. Martin

doi:10.1117/12.2181040

19 June 2015 Confocal Raman study of aging process in diabetes mellitus human voluntaries

Liliane Pereira, Claudio Alberto Téllez Soto, Laurita dos Santos, Syed Mohammed Ali, Priscila Pereira Fávero, Airton A. Martin

Author Affiliations +

Proceedings Volume 9531, Biophotonics South America; 95312M (2015) https://doi.org/10.1117/12.2181040
Event: SPIE Biophotonics South America, 2015, Rio de Janeiro, Brazil

Abstract

Accumulation of AGEs [Advanced Glycation End – products] occurs slowly during the human aging process. However, its formation is accelerated in the presence of diabetes mellitus. In this paper, we perform a noninvasive analysis of glycation effect on human skin by in vivo confocal Raman spectroscopy. This technique uses a laser of 785 nm as excitation source and, by the inelastic scattering of light, it is possible to obtain information about the biochemical composition of the skin. Our aim in this work was to characterize the aging process resulting from the glycation process in a group of 10 Health Elderly Women (HEW) and 10 Diabetic Elderly Women (DEW). The Raman data were collected from the dermis at a depth of 70-130 microns. Through the theory of functional density (DFT) the bands positions of hydroxyproline, proline and AGEs (pentosidine and glucosepane) were calculated by using Gaussian 0.9 software. A molecular interpretation of changes in type I collagen was performed by the changes in the vibrational modes of the proline (P) and hydroxyproline (HP). The data analysis shows that the aging effects caused by glycation of proteins degrades type I collagen differently and leads to accelerated aging process.

Citation Download Citation

Liliane Pereira, Claudio Alberto Téllez Soto, Laurita dos Santos, Syed Mohammed Ali, Priscila Pereira Fávero, and Airton A. Martin "Confocal Raman study of aging process in diabetes mellitus human voluntaries", Proc. SPIE 9531, Biophotonics South America, 95312M (19 June 2015); https://doi.org/10.1117/12.2181040

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