Laser speckle contrast imaging of blood flow from anesthetized mice: correcting drifts in measurements due to breathing movements

Gesse E. C. Nogueira; Márcio A. C. Ribeiro; Juliane C. Campos; Julio C. B. Ferreira

doi:10.1117/12.2087063

19 June 2015 Laser speckle contrast imaging of blood flow from anesthetized mice: correcting drifts in measurements due to breathing movements

Gesse E. C. Nogueira, Márcio A. C. Ribeiro, Juliane C. Campos, Julio C. B. Ferreira

Author Affiliations +

Proceedings Volume 9531, Biophotonics South America; 95314G (2015) https://doi.org/10.1117/12.2087063
Event: SPIE Biophotonics South America, 2015, Rio de Janeiro, Brazil

Abstract

Background: Laser speckle contrast imaging allows non-invasive assessment of cutaneous blood flow. Although the technique is attractive to measure a quantity related to the skin blood flow (SBF) in anesthetized animal models, movements from breathing can mask the SBF signal. As a consequence, the measurement is overestimated because a variable amount of a DC component due to the breathing movements is added to the SBF signal. Objective: To evaluate a method for estimating the background level of the SBF signal, rejecting artefacts from breathing. Methods: A baseline correction method used for accurate DNA sequencing was evaluated, based on estimating the background level of a signal in small temporal sliding-windows. The method was applied to evaluate a mouse model of hindlimb ischemia. SBF signals from hindlimbs of anesthetized C57BL/6 mice (n=13) were registered. The mean SBF (Fi and Fc from ischemic and control hindlimbs) were computed from the registers and from the corresponding estimated background levels (Fib and Fcb from ischemic and control hindlimbs). Results: The mean values of the percentages (a measure of ischemia) MI = (Fi/Fc).100 and MIb = (Fib/Fcb).100 were computed to be 30±4% and 23±3% respectively (mean ± SE). Evidences of statistical differences between both, ischemic and control hindlimbs, were obtained (p<0.05, paired student-t). The mean error [(MI-MIb)/MIb].100 obtained was 45±14% (mean±SE). Conclusion: The recovery of a corrupted SBF signal by breathing artefacts is feasible, allowing more accurate measurements.

Citation Download Citation

Gesse E. C. Nogueira, Márcio A. C. Ribeiro, Juliane C. Campos, and Julio C. B. Ferreira "Laser speckle contrast imaging of blood flow from anesthetized mice: correcting drifts in measurements due to breathing movements", Proc. SPIE 9531, Biophotonics South America, 95314G (19 June 2015); https://doi.org/10.1117/12.2087063

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
6 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Blood circulation

Laser speckle contrast imaging

Ischemia

Error analysis

Signal processing

Skin

Biological research

Show All Keywords

Keywords/Phrases

Search In:

Publication Years