Accurate and in situ monitoring of bacterial concentration using a real time all-fibre spectroscopic device

W. Tao; C. McGoverin; S. Lydiard; Y. Song; M. Cheng; S. Swift; N. Singhal; F. Vanholsbeeck

doi:10.1117/12.2184388

1 July 2015 Accurate and in situ monitoring of bacterial concentration using a real time all-fibre spectroscopic device

W. Tao, C. McGoverin, S. Lydiard, Y. Song, M. Cheng, S. Swift, N. Singhal, F. Vanholsbeeck

Proceedings Volume 9655, Fifth Asia-Pacific Optical Sensors Conference; 96551J (2015) https://doi.org/10.1117/12.2184388
Event: Fifth Asia Pacific Optical Sensors Conference, 2015, Jeju, Korea, Republic of

Abstract

Accurate in situ monitoring of bacterial transport is important for increased understanding and improvement of bioremediation processes where microorganisms convert toxic compounds to more benign compounds. Bioremediation methods have become the preferred mechanism for the rehabilitation of hard to reach contaminated environments. In this study, we have used fluorescence spectroscopy to monitor the movement of fluorescently labelled bacteria (Rhodococcus erythropolis and Pseudomonas putida) within a bench-top column filled with a porous medium. In situ fluorescence measurements made using a fibre optic based instrument (‘optrode’) were compared to ex situ measurements made using a plate reader. In situ monitoring using this fibre optic based instrument is a promising alternative to ex situ measurements as the initial flow of bacteria is reliably observed. However, a greater understanding of the effect of the porous medium on fluorescence measurements is required to develop an accurate calibration for bacterial concentration based in situ measurements.

Citation Download Citation

W. Tao, C. McGoverin, S. Lydiard, Y. Song, M. Cheng, S. Swift, N. Singhal, and F. Vanholsbeeck "Accurate and in situ monitoring of bacterial concentration using a real time all-fibre spectroscopic device", Proc. SPIE 9655, Fifth Asia-Pacific Optical Sensors Conference, 96551J (1 July 2015); https://doi.org/10.1117/12.2184388

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