Laser surface treatment of polyamide and NiTi alloy and the effects on mesenchymal stem cell response

D. G. Waugh; J. Lawrence; P. Shukla; C. Chan; I. Hussain; H. C. Man; G. C. Smith

doi:10.1117/12.2182432

1 July 2015 Laser surface treatment of polyamide and NiTi alloy and the effects on mesenchymal stem cell response

D. G. Waugh, J. Lawrence, P. Shukla, C. Chan, I. Hussain, H. C. Man, G. C. Smith

Proceedings Volume 9657, Industrial Laser Applications Symposium (ILAS 2015); 96570L (2015) https://doi.org/10.1117/12.2182432
Event: Industrial Laser Applications Symposium 2015, 2015, Kenilworth, United Kingdom

Abstract

Mesenchymal stem cells (MSCs) are known to play important roles in development, post-natal growth, repair, and regeneration of mesenchymal tissues. What is more, surface treatments are widely reported to affect the biomimetic nature of materials. This paper will detail, discuss and compare laser surface treatment of polyamide (Polyamide 6,6), using a 60 W CO₂ laser, and NiTi alloy, using a 100 W fiber laser, and the effects of these treatments on mesenchymal stem cell response. The surface morphology and composition of the polyamide and NiTi alloy were studied by scanning electron microscopy (SEM) and X-ray photoemission spectroscopy (XPS), respectively. MSC cell morphology cell counting and viability measurements were done by employing a haemocytometer and MTT colorimetric assay. The success of enhanced adhesion and spreading of the MSCs on each of the laser surface treated samples, when compared to as-received samples, is evidenced in this work.

Citation Download Citation

D. G. Waugh, J. Lawrence, P. Shukla, C. Chan, I. Hussain, H. C. Man, and G. C. Smith "Laser surface treatment of polyamide and NiTi alloy and the effects on mesenchymal stem cell response", Proc. SPIE 9657, Industrial Laser Applications Symposium (ILAS 2015), 96570L (1 July 2015); https://doi.org/10.1117/12.2182432

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