A simple gel electrophoresis method for separating polyhedral gold nanoparticles

Suhee Kim; Hye Jin Lee

doi:10.1117/12.2189772

8 July 2015 A simple gel electrophoresis method for separating polyhedral gold nanoparticles

Suhee Kim, Hye Jin Lee

Proceedings Volume 9523, International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2015; 95230B (2015) https://doi.org/10.1117/12.2189772
Event: International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2015, 2015, Jeju, Korea, Republic of

Abstract

In this paper, a simple approach to separate differently shaped and sized polyhedral gold nanoparticles (NPs) within colloidal solutions via gel electrophoresis is described. Gel running parameters for separating efficiently gold NPs including gel composition, added surfactant types and applied voltage were investigated. The plasmonic properties and physical structure of the separated NPs extracted from the gel matrix were then investigated using transmission electron microscopy (TEM) and UV-vis spectrophotometry respectively. Data analysis revealed that gel electrophoresis conditions of a 1.5 % agarose gel with 0.1 % sodium dodecyl sulfate (SDS) surfactant under an applied voltage of 100 V resulted in the selective isolation of ~ 50 nm polyhedral shaped gold nanoparticles. Further efforts are underway to apply the method to purify biomolecule-conjugated polyhedral Au NPs that can be readily used for NP-enhanced biosensing platforms.

Citation Download Citation

Suhee Kim and Hye Jin Lee "A simple gel electrophoresis method for separating polyhedral gold nanoparticles", Proc. SPIE 9523, International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2015, 95230B (8 July 2015); https://doi.org/10.1117/12.2189772

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KEYWORDS

Nanoparticles

Gold

Transmission electron microscopy

Sodium

Biosensing

Spherical lenses

Imaging spectroscopy

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