Raman spectroscopy of stored red blood cells: evaluating clinically-relevant biochemical markers in donated blood

Chad G. Atkins; Kevin Buckley; Deborah Chen; H. Georg Schulze; Dana V. Devine; Michael W. Blades; Robin F. B. Turner

doi:10.1117/12.2183598

15 July 2015 Raman spectroscopy of stored red blood cells: evaluating clinically-relevant biochemical markers in donated blood

Chad G. Atkins, Kevin Buckley, Deborah Chen, H. Georg Schulze, Dana V. Devine, Michael W. Blades, Robin F. B. Turner

Author Affiliations +

Proceedings Volume 9537, Clinical and Biomedical Spectroscopy and Imaging IV; 95370X (2015) https://doi.org/10.1117/12.2183598
Event: European Conferences on Biomedical Optics, 2015, Munich, Germany

Abstract

Modern transfusion medicine relies on the safe, secure, and cost-effective delivery of donated red blood cells (RBCs). Once isolated, RBCs are suspended in a defined additive solution and stored in plastic blood bags in which, over time, they undergo chemical, physiological, and morphological changes that may have a deleterious impact on some patients. Regulations limit the storage period to 42 days and the cells do not routinely undergo analytical testing before use. In this study, we use Raman spectroscopy to interrogate stored RBCs and we identify metabolic and cell-breakdown products, such as haemoglobin and membrane fragments, that build-up in the blood bags as the cells age. Our work points the way to the development of an instrument which could quickly and easily assess the biochemical nature of stored RBC units before they are transfused.

Citation Download Citation

Chad G. Atkins, Kevin Buckley, Deborah Chen, H. Georg Schulze, Dana V. Devine, Michael W. Blades, and Robin F. B. Turner "Raman spectroscopy of stored red blood cells: evaluating clinically-relevant biochemical markers in donated blood", Proc. SPIE 9537, Clinical and Biomedical Spectroscopy and Imaging IV, 95370X (15 July 2015); https://doi.org/10.1117/12.2183598

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