CONFERENCE PROCEEDINGS
Advanced Search
Home > Proceedings > Volume 9536 > Article
Paper
16 July 2015 Lensfree video microscopy: high throughput monitoring and cell tracking of 2D cell cultures
C. P. Allier, S. Vinjimore Kesavan, O. Cioni, F. Momey, T. Bordy, L. Hervé, S. Morel, F. Navarro, M. Menneteau, B. Chalmond, D. Freida, E. Sulpice, X. Gidrol, J.-M. Dinten
Author Affiliations +
Proceedings Volume 9536, Advanced Microscopy Techniques IV; and Neurophotonics II; 95360J (2015) https://doi.org/10.1117/12.2183468
Event: European Conferences on Biomedical Optics, 2015, Munich, Germany
Abstract
In order to extend the analysis of the datasets produced by lensfree video microscopy we have implemented a cell tracking algorithm to combine and correlate cell motility to the previously devised metrics to quantify e.g. cell adhesion and spreading, cell division, and cell death. In this paper we present the assessment of these new methodology on experiments involving three different cell lines, namely 3T3 fibroblast cells, primary HUVEC cells and macrophage THP1 cells. We demonstrate that the good spatial resolution and the fast frame rate obtained with of our lensfree video microscope allows standard cell tracking algorithm to be computed. The results is the possibility to analyze thousands of cells successfully tracked over tens of hours. The results is the possibility to compare different cell cultures in terms of e.g. cell motility and cell confinement ration. Ultimately we managed to measure the doubling time at single cell level over a large number of N=235 cells tracked over two days.
© (2015) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation Download Citation
C. P. Allier, S. Vinjimore Kesavan, O. Cioni, F. Momey, T. Bordy, L. Hervé, S. Morel, F. Navarro, M. Menneteau, B. Chalmond, D. Freida, E. Sulpice, X. Gidrol, and J.-M. Dinten "Lensfree video microscopy: high throughput monitoring and cell tracking of 2D cell cultures", Proc. SPIE 9536, Advanced Microscopy Techniques IV; and Neurophotonics II, 95360J (16 July 2015); https://doi.org/10.1117/12.2183468
ACCESS THE FULL ARTICLE
ORGANIZATIONAL
Sign in with credentials provided by your organization.
INSTITUTIONAL
Select your institution to access the SPIE Digital Library.
SELECT YOUR INSTITUTION
PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
PERSONAL SIGN IN
No SPIE Account? Create one
PURCHASE THIS CONTENT
SUBSCRIBE TO DIGITAL LIBRARY
50 downloads per 1-year subscription
Members: $195
Non-members: $335 ADD TO CART
25 downloads per 1 - year subscription
Members: $145
Non-members: $250 ADD TO CART
PURCHASE SINGLE ARTICLE
Includes PDF, HTML & Video, when available
Members: $17.00
Non-members: $21.00 ADD TO CART
PROCEEDINGS
 10 PAGES
DOWNLOAD PAPER SAVE TO MY LIBRARY
GET CITATION
Advertisement
Advertisement
RIGHTS & PERMISSIONS
Get copyright permission  Get copyright permission on Copyright Marketplace
KEYWORDS
Detection and tracking algorithms
Video microscopy
Image segmentation
Video
Microscopes
Signal to noise ratio
Holography
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top