Integrated 3D macro-trapping and light-sheet imaging system

Zhengyi Yang; Peeter Piksarv; David E. K. Ferrier; Frank J. Gunn-Moore; Kishan Dholakia

doi:10.1117/12.2186399

25 August 2015 Integrated 3D macro-trapping and light-sheet imaging system

Zhengyi Yang, Peeter Piksarv, David E. K. Ferrier, Frank J. Gunn-Moore, Kishan Dholakia

Proceedings Volume 9548, Optical Trapping and Optical Micromanipulation XII; 95480T (2015) https://doi.org/10.1117/12.2186399
Event: SPIE Nanoscience + Engineering, 2015, San Diego, California, United States

Abstract

Biological research requires high-speed and low-damage imaging techniques for live specimens in areas such as development study in embryos. Light sheet microscopy provides fast imaging speed whilst keeps the photo-damage and photo-blenching to minimum. Conventional sample embedding methods in light sheet imaging involves using agent such as agarose which potentially affects the behavior and the develop pattern of the specimens. Here we demonstrate integrating dual-beam trapping method into light sheet imaging system to confine and translate the specimen whilst light sheet images are taken. Tobacco plant cells as well as Spirobranchus lamarcki larva were trapped solely with optical force and sectional images were acquired. This now approach has the potential to extend the applications of light sheet imaging significantly.

Citation Download Citation

Zhengyi Yang, Peeter Piksarv, David E. K. Ferrier, Frank J. Gunn-Moore, and Kishan Dholakia "Integrated 3D macro-trapping and light-sheet imaging system", Proc. SPIE 9548, Optical Trapping and Optical Micromanipulation XII, 95480T (25 August 2015); https://doi.org/10.1117/12.2186399

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