Quantitative high spatiotemporal imaging of biological processes

Joe Borbely; Jason Otterstrom; Nitin Mohan; Carlo Manzo; Melike Lakadamyali

doi:10.1117/12.2190263

26 August 2015 Quantitative high spatiotemporal imaging of biological processes

Joe Borbely, Jason Otterstrom, Nitin Mohan, Carlo Manzo, Melike Lakadamyali

Proceedings Volume 9554, Nanoimaging and Nanospectroscopy III; 95540M (2015) https://doi.org/10.1117/12.2190263
Event: SPIE Nanoscience + Engineering, 2015, San Diego, California, United States

Abstract

Super-resolution microscopy has revolutionized fluorescence imaging providing access to length scales that are much below the diffraction limit. The super-resolution methods have the potential for novel discoveries in biology. However, certain technical limitations must be overcome for this potential to be fulfilled. One of the main challenges is the use of super-resolution to study dynamic events in living cells. In addition, the ability to extract quantitative information from the super-resolution images is confounded by the complex photophysics that the fluorescent probes exhibit during the imaging. Here, we will review recent developments we have been implementing to overcome these challenges and introduce new steps in automated data acquisition towards high-throughput imaging.

Conference Presentation

Citation Download Citation

Joe Borbely, Jason Otterstrom, Nitin Mohan, Carlo Manzo, and Melike Lakadamyali "Quantitative high spatiotemporal imaging of biological processes", Proc. SPIE 9554, Nanoimaging and Nanospectroscopy III, 95540M (26 August 2015); https://doi.org/10.1117/12.2190263

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