Detection specificity studies of bacteriophage adhesin-coated long-period grating-based biosensor

Marcin Koba; Mateusz Śmietana; Ewa Brzozowska; Sabina Górska; Predrag Mikulic; Andrea Cusano; Wojtek J. Bock

doi:10.1117/12.2194467

28 September 2015 Detection specificity studies of bacteriophage adhesin-coated long-period grating-based biosensor

Marcin Koba, Mateusz Śmietana, Ewa Brzozowska, Sabina Górska, Predrag Mikulic, Andrea Cusano, Wojtek J. Bock

Proceedings Volume 9634, 24th International Conference on Optical Fibre Sensors; 963426 (2015) https://doi.org/10.1117/12.2194467
Event: International Conference on Optical Fibre Sensors (OFS24), 2015, Curitiba, Brazil

Abstract

In this work, we present a label-free detection specificity study of an optical fiber long-period grating (LPG) biosensor working near the dispersion turning point of higher order cladding modes. The LPG sensor functionalized with bacteriophage adhesin is tested with specific and non-specific bacteria dry weight. We show that such biosensor is able to selectively bind, thus recognize different bacteria. We use bacteria dry weights of E. coli B as positive test and E. coli K12 and Salmonella enterica as negative tests. The resonance wavelength shift induced by E. coli B reaches over 90 nm, while for E. coli K12 and Salmonella enterica approximately 40 and 20 nm, respectively.

Citation Download Citation

Marcin Koba, Mateusz Śmietana, Ewa Brzozowska, Sabina Górska, Predrag Mikulic, Andrea Cusano, and Wojtek J. Bock "Detection specificity studies of bacteriophage adhesin-coated long-period grating-based biosensor", Proc. SPIE 9634, 24th International Conference on Optical Fibre Sensors, 963426 (28 September 2015); https://doi.org/10.1117/12.2194467

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