Stabilization of glucose-oxidase in the graphene paste for screen-printed glucose biosensor

Andrzej Pepłowski; Daniel Janczak; Małgorzata Jakubowska

doi:10.1117/12.2205830

11 September 2015 Stabilization of glucose-oxidase in the graphene paste for screen-printed glucose biosensor

Andrzej Pepłowski, Daniel Janczak, Małgorzata Jakubowska

Proceedings Volume 9662, Photonics Applications in Astronomy, Communications, Industry, and High-Energy Physics Experiments 2015; 96624H (2015) https://doi.org/10.1117/12.2205830
Event: XXXVI Symposium on Photonics Applications in Astronomy, Communications, Industry, and High-Energy Physics Experiments (Wilga 2015), 2015, Wilga, Poland

Abstract

Various methods and materials for enzyme stabilization within screen-printed graphene sensor were analyzed. Main goal was to develop technology allowing immediate printing of the biosensors in single printing process. Factors being considered were: toxicity of the materials used, ability of the material to be screen-printed (squeezed through the printing mesh) and temperatures required in the fabrication process. Performance of the examined sensors was measured using chemical amperometry method, then appropriate analysis of the measurements was conducted. The analysis results were then compared with the medical requirements. Parameters calculated were: correlation coefficient between concentration of the analyte and the measured electrical current (0.986) and variation coefficient for the particular concentrations of the analyte used as the calibration points. Variation of the measured values was significant only in ranges close to 0, decreasing for the concentrations of clinical importance. These outcomes justify further development of the graphene-based biosensors fabricated through printing techniques.

Citation Download Citation

Andrzej Pepłowski, Daniel Janczak, and Małgorzata Jakubowska "Stabilization of glucose-oxidase in the graphene paste for screen-printed glucose biosensor", Proc. SPIE 9662, Photonics Applications in Astronomy, Communications, Industry, and High-Energy Physics Experiments 2015, 96624H (11 September 2015); https://doi.org/10.1117/12.2205830

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