Multicolor fluorescence microscopic imaging of cancer cells on the plasmonic chip (Presentation Recording)

Keiko Tawa; Chisato Sasakawa; Shohei Yamamura; Izumi Shibata; Masatoshi Kataoka

doi:10.1117/12.2186276

5 October 2015 Multicolor fluorescence microscopic imaging of cancer cells on the plasmonic chip (Presentation Recording)

Keiko Tawa, Chisato Sasakawa, Shohei Yamamura, Izumi Shibata, Masatoshi Kataoka

Author Affiliations +

Proceedings Volume 9547, Plasmonics: Metallic Nanostructures and Their Optical Properties XIII; 95470I (2015) https://doi.org/10.1117/12.2186276
Event: SPIE Nanoscience + Engineering, 2015, San Diego, California, United States

Abstract

A plasmonic chip which is a metal coated substrate with grating structure can provide the enhanced fluorescence by the grating-coupled surface plasmon field. In our previous studies, bright epi-fluorescence microscopic imaging of neuron cells and sensitive immunosesnsing have been reported. In this study, two kinds of breast cancer cells, MCF-7 and MDA-MB231, were observed with epi-fluorescence microscope on the plasmonic chip with 2D hole-arrays . They were multicolor stained with 4', 6-diamidino-2-phenylindole (DAPI) and allophycocyanin (APC)-labeled anti-epithelial cell adhesion molecule (EpCAM) antibody. Our plasmonic chip provided the brighter fluorescence images of these cells compared with the glass slide. Even in the cells including few EpCAM, the distribution of EpCAM was clearly observed in the cell membrane. It was found that the plasmonic chip can be one of the powerful tools to detect the marker protein existing around the chip surface even at low concentration.

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Citation Download Citation

Keiko Tawa, Chisato Sasakawa, Shohei Yamamura, Izumi Shibata, and Masatoshi Kataoka "Multicolor fluorescence microscopic imaging of cancer cells on the plasmonic chip (Presentation Recording)", Proc. SPIE 9547, Plasmonics: Metallic Nanostructures and Their Optical Properties XIII, 95470I (5 October 2015); https://doi.org/10.1117/12.2186276

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KEYWORDS

Plasmonics

Luminescence

Cancer

Breast cancer

Glasses

Metals

Microscopes

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