Enhanced in-vivo optical coherence tomography of live mouse brain by the use of implanted micro-lens (Presentation Recording)

Iman Hassani Nia; Daniel Dombeck; Hooman Mohseni

doi:10.1117/12.2187642

5 October 2015 Enhanced in-vivo optical coherence tomography of live mouse brain by the use of implanted micro-lens (Presentation Recording)

Iman Hassani Nia, Daniel Dombeck, Hooman Mohseni

Author Affiliations +

Proceedings Volume 9550, Biosensing and Nanomedicine VIII; 955005 (2015) https://doi.org/10.1117/12.2187642
Event: SPIE Nanoscience + Engineering, 2015, San Diego, California, United States

Abstract

Near-infrared optical coherence tomography (OCT) has gained a lot of attention due to the fact that it is relatively cheap, non-invasive and provides high resolution and fast method of imaging. However the main challenge of this technique is the poor signal to noise ratio of the images of the tissue at large depths due to optical scattering. The signal to noise ratio can be improved by increasing the source power, however the laser safety standards (ANSI Z136.1) restricts the maximum amount of power that can be used safely to characterize the biological tissue. In this talk, we discuss the advantage of implanting a micro-lens inside the tissue to have a higher signal to noise ratio for confocal and OCT measurements. We explain the theoretical background, experimental setup and the method of implanting the micro lens at arbitrary depths within a live mouse brain. The in-vivo 3D OCT and two-photon microscopy images of live mouse with implanted micro-lens are presented and significant enhancement of signal to noise ratio is observed. The confocal and OCT measurements have been performed with super-luminescent LEDs emitting at 1300 nm. We believe that the high resolution and high sensitivity of this technique is of fundamental importance for characterization of neural activity, monitoring the hemodynamic responses, tumors and for performing image guided surgeries.

Conference Presentation

Citation Download Citation

Iman Hassani Nia, Daniel Dombeck, and Hooman Mohseni "Enhanced in-vivo optical coherence tomography of live mouse brain by the use of implanted micro-lens (Presentation Recording)", Proc. SPIE 9550, Biosensing and Nanomedicine VIII, 955005 (5 October 2015); https://doi.org/10.1117/12.2187642

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

Optical coherence tomography

Signal to noise ratio

Brain

In vivo imaging

Confocal microscopy

Image resolution

Tissue optics

Show All Keywords

Keywords/Phrases

Search In:

Publication Years