The numerical simulation and goniometric measurements of cells light scattering based on Mie theory

Xiaogang Lin; Changbin Ye; Hao Zhu; Wenchao Li; Jie Wu

doi:10.1117/12.2202917

15 October 2015 The numerical simulation and goniometric measurements of cells light scattering based on Mie theory

Xiaogang Lin, Changbin Ye, Hao Zhu, Wenchao Li, Jie Wu

Proceedings Volume 9672, AOPC 2015: Advanced Display Technology; and Micro/Nano Optical Imaging Technologies and Applications; 967211 (2015) https://doi.org/10.1117/12.2202917
Event: Applied Optics and Photonics China (AOPC2015), 2015, Beijing, China

Abstract

Optical diagnostic technique, due to its rapid and non-invasive for the diagnosis diseases at the cellular level, can be performed in vivo and allow for real-time diagnosis. While light scattering method is capable of characterizing the structural properties of tissue at the cellular and subcellular scale. In this paper, the spherical models of cells light scattering were established based on Mie, and the distribution curves of scattering intensity in the range of 0~180 degrees were got to explore change rule of cells light scattering information at the molecular level. Also, a platform for experiments used to measure the light scattering information of cells was built to get the change rule of cells light scattering information in wide angular range. And the particle size distribution (PSD) of cells was got by the inversion algorithm. A comparative analysis between numerical simulation and goniometric measurements revealed that the forward-scattering and side-scattering were influenced by the particle size of cells and relative index of refraction between cells and surrounding media. It could also be concluded that it was necessary to get and analyze the light scattering information of larger scattering angle range, which may be related to the intracellular organelles and nucleus.

Citation Download Citation

Xiaogang Lin, Changbin Ye, Hao Zhu, Wenchao Li, and Jie Wu "The numerical simulation and goniometric measurements of cells light scattering based on Mie theory", Proc. SPIE 9672, AOPC 2015: Advanced Display Technology; and Micro/Nano Optical Imaging Technologies and Applications, 967211 (15 October 2015); https://doi.org/10.1117/12.2202917

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
6 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Light scattering

Scattering

Mie scattering

Particles

Spherical lenses

Numerical simulations

Tissue optics

Show All Keywords

Keywords/Phrases

Search In:

Publication Years