Different strategies for the detection of bioagents using electrochemical and photoelectrochemical genosensors

Diego Voccia; Francesca Bettazzi; Ilaria Palchetti

doi:10.1117/12.2194838

21 October 2015 Different strategies for the detection of bioagents using electrochemical and photoelectrochemical genosensors

Diego Voccia, Francesca Bettazzi, Ilaria Palchetti

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Proceedings Volume 9652, Optics and Photonics for Counterterrorism, Crime Fighting, and Defence XI; and Optical Materials and Biomaterials in Security and Defence Systems Technology XII; 96520T (2015) https://doi.org/10.1117/12.2194838
Event: SPIE Security + Defence, 2015, Toulouse, France

Abstract

In recent years various kinds of biosensors for the detection of pathogens have been developed. A genosensor consists in the immobilization, onto the surface of a chosen transducer, of an oligonucleotide with a specific base sequence called capture probe. The complementary sequence (the analytical target, i.e. a specific sequence of the DNA/RNA of the pathogen) present in the sample is recognized and captured by the probe through the hybridization reaction. The evaluation of the extent of the hybridization allows one to confirm whether the sample contains the complementary sequence of the probe or not. Electrochemical transducers have received considerable attention in connection with the detection of DNA hybridization. Moreover, recently, with the emergence of novel photoelectrochemically active species and new detection schemes, photoelectrochemistry has resulted in substantial progress in its analytical performance for biosensing applications. In this paper, some examples of electrochemical genosensors for multiplexed pathogen detection are shown. Moreover, the preliminary experiments towards the development of a photoelectrochemical genosensor using a TiO2 – nanocrystal-modified ITO electrode are discussed.

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Diego Voccia, Francesca Bettazzi, and Ilaria Palchetti "Different strategies for the detection of bioagents using electrochemical and photoelectrochemical genosensors", Proc. SPIE 9652, Optics and Photonics for Counterterrorism, Crime Fighting, and Defence XI; and Optical Materials and Biomaterials in Security and Defence Systems Technology XII, 96520T (21 October 2015); https://doi.org/10.1117/12.2194838

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KEYWORDS

Electrodes

Pathogens

Titanium dioxide

Biosensors

Transducers

Multiplexing

Statistical analysis

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