Non-interferometric quantitative phase imaging of yeast cells

Praveen Kumar Poola; Vimal Prabhu Pandiyan; Renu John

doi:10.1117/12.2207232

9 December 2015 Non-interferometric quantitative phase imaging of yeast cells

Praveen Kumar Poola, Vimal Prabhu Pandiyan, Renu John

Proceedings Volume 9792, Biophotonics Japan 2015; 97920G (2015) https://doi.org/10.1117/12.2207232
Event: SPIE/OSJ Biophotonics Japan, 2015, Tokyo, Japan

Abstract

Real-time imaging of live cells is quite difficult without the addition of external contrast agents. Various methods for quantitative phase imaging of living cells have been proposed like digital holographic microscopy and diffraction phase microscopy. In this paper, we report theoretical and experimental results of quantitative phase imaging of live yeast cells with nanometric precision using transport of intensity equations (TIE). We demonstrate nanometric depth sensitivity in imaging live yeast cells using this technique. This technique being noninterferometric, does not need any coherent light sources and images can be captured through a regular bright-field microscope. This real-time imaging technique would deliver the depth or 3-D volume information of cells and is highly promising in real-time digital pathology applications, screening of pathogens and staging of diseases like malaria as it does not need any preprocessing of samples.

Citation Download Citation

Praveen Kumar Poola, Vimal Prabhu Pandiyan, and Renu John "Non-interferometric quantitative phase imaging of yeast cells", Proc. SPIE 9792, Biophotonics Japan 2015, 97920G (9 December 2015); https://doi.org/10.1117/12.2207232

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