Study on fluorescence spectra of thiamine, riboflavin and pyridoxine

Hui Yang; Xue Xiao; Xuesong Zhao; Lan Hu; Caofang Lv; Zhangkun Yin

doi:10.1117/12.2211248

26 January 2016 Study on fluorescence spectra of thiamine, riboflavin and pyridoxine

Hui Yang, Xue Xiao, Xuesong Zhao, Lan Hu, Caofang Lv, Zhangkun Yin

Proceedings Volume 9903, Seventh International Symposium on Precision Mechanical Measurements; 99030H (2016) https://doi.org/10.1117/12.2211248
Event: Seventh International Symposium on Precision Mechanical Measurements, 2015, Xia'men, China

Abstract

This paper presents the intrinsic fluorescence characteristics of vitamin B1, B2 and B6 measured with 3D fluorescence Spectrophotometer. Three strong fluorescence areas of vitamin B2 locate at λex/λem=270/525nm, 370/525nm and 450/525nm, one fluorescence areas of vitamin B1 locates at λex/λem=370/460nm, two fluorescence areas of vitamin B6 locate at λex/λem=250/370nm and 325/370nm were found. The influence of pH of solution to the fluorescence profile was also discussed. Using the PARAFAC algorithm, 10 vitamin B1, B2 and B6 mixed solutions were successfully decomposed, and the emission profiles, excitation profiles, central wavelengths and the concentration of the three components were retrieved precisely through about 5 iteration times.

Citation Download Citation

Hui Yang, Xue Xiao, Xuesong Zhao, Lan Hu, Caofang Lv, and Zhangkun Yin "Study on fluorescence spectra of thiamine, riboflavin and pyridoxine", Proc. SPIE 9903, Seventh International Symposium on Precision Mechanical Measurements, 99030H (26 January 2016); https://doi.org/10.1117/12.2211248

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