Potential of mid IR spectroscopy in the rapid label free identification of skin malignancies

Lena Kastl; Björn Kemper; Gavin R. Lloyd; Jayakrupakar Nallala; Nick Stone; Valery Naranjo; Francisco Penaranda; Jürgen Schnekenburger

doi:10.1117/12.2208897

7 March 2016 Potential of mid IR spectroscopy in the rapid label free identification of skin malignancies

Lena Kastl, Björn Kemper, Gavin R. Lloyd, Jayakrupakar Nallala, Nick Stone, Valery Naranjo, Francisco Penaranda, Jürgen Schnekenburger

Author Affiliations +

Proceedings Volume 9703, Optical Biopsy XIV: Toward Real-Time Spectroscopic Imaging and Diagnosis; 970307 (2016) https://doi.org/10.1117/12.2208897
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

The rapid inspection of suspicious skin lesions for pathological cell types is the objective of optical point of care diagnostics technologies. A marker free fast diagnosis of skin malignancies would overcome the limitations of the current gold standard surgical biopsy. The time consuming and costly biopsy procedure requires the inspection of each sample by a trained pathologist, which limits the analysis of potentially malignant lesions. Optical technologies like RAMAN or infrared spectroscopy, which provide both, localization and chemical information, can be used to differentiate malignant from healthy tissue by the analysis of multi cell structures and cell type specific spectra. We here report the application of midIR spectroscopy towards fast and reliable skin diagnostics. Within the European research project MINERVA we developed standardized in vitro skin systems with increasing complexity, from single skin cell types as fibroblasts, keratinocytes and melanoma cells, to mixtures of these and finally three dimensional human skin equivalents. The standards were characterized in the established midIR range and also with newly developed systems for fast imaging up to 12 μm. The analysis of the spectra by novel data processing algorithms demonstrated the clear separation of all cell types, especially the tumor cells. The signals from single cell layers were sufficient for cell type differentiation. We have compared different midIR systems and found all of them suitable for specific cell type identification. Our data demonstrate the potential of midIR spectroscopy for fast image acquisition and an improved data processing as sensitive and specific optical biopsy technology.

Conference Presentation

Citation Download Citation

Lena Kastl, Björn Kemper, Gavin R. Lloyd, Jayakrupakar Nallala, Nick Stone, Valery Naranjo, Francisco Penaranda, and Jürgen Schnekenburger "Potential of mid IR spectroscopy in the rapid label free identification of skin malignancies", Proc. SPIE 9703, Optical Biopsy XIV: Toward Real-Time Spectroscopic Imaging and Diagnosis, 970307 (7 March 2016); https://doi.org/10.1117/12.2208897

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