Preliminary investigation of intrinsic UV fluorescence spectroscopic changes associated with proteolytic digestion of bovine articular cartilage

William Lewis; Juan-Pablo Padilla-Martinez; Antonio Ortega-Martinez; Walfre Franco

doi:10.1117/12.2212932

7 March 2016 Preliminary investigation of intrinsic UV fluorescence spectroscopic changes associated with proteolytic digestion of bovine articular cartilage

William Lewis, Juan-Pablo Padilla-Martinez, Antonio Ortega-Martinez, Walfre Franco

Author Affiliations +

Proceedings Volume 9703, Optical Biopsy XIV: Toward Real-Time Spectroscopic Imaging and Diagnosis; 97031B (2016) https://doi.org/10.1117/12.2212932
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Degradation and destruction of articular cartilage is the etiology of osteoarthritis (OA), an entity second only to cardiovascular disease as a cause of disability in the United States. Joint mechanics and cartilage biochemistry are believed to play a role in OA; an optical tool to detect structural and chemical changes in articular cartilage might offer benefit for its early detection and treatment. The objective of the present study was to identify the spectral changes in intrinsic ultraviolet (UV) fluorescence of cartilage that occur after proteolytic digestion of cartilage. Bovine articular cartilage samples were incubated in varying concentrations of collagenase ranging from 10ug/mL up to 5mg/mL for 18 hours at 37°C, a model of OA. Pre- and post-incubation measurements were taken of the UV excitation-emission spectrum of each cartilage sample. Mechanical tests were performed to determine the pre- and post-digestion force/displacement ratio associated with indentation of each sample. Spectral changes in intrinsic cartilage fluorescence and stiffness of the cartilage were associated with proteolytic digestion. In particular, changes in the relative intensity of fluorescence peaks associated with pentosidine crosslinks (330 nm excitation, 390 nm emission) and tryptophan (290 nm excitation, 340 nm emission) were found to correlate with different degrees of cartilage digestion and cartilage stiffness. In principle, it may be possible to use UV fluorescence spectral data for early detection of damage to articular cartilage, and as a surrogate measure for cartilage stiffness.

Conference Presentation

Citation Download Citation

William Lewis, Juan-Pablo Padilla-Martinez, Antonio Ortega-Martinez, and Walfre Franco "Preliminary investigation of intrinsic UV fluorescence spectroscopic changes associated with proteolytic digestion of bovine articular cartilage", Proc. SPIE 9703, Optical Biopsy XIV: Toward Real-Time Spectroscopic Imaging and Diagnosis, 97031B (7 March 2016); https://doi.org/10.1117/12.2212932

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