Extremely sensitive dual imaging system in solid phantoms

Eran A. Barnoy; Dror Fixler; Rachela Popovtzer; Tsviya Nayhoz; Krishanu Ray

doi:10.1117/12.2207908

22 April 2016 Extremely sensitive dual imaging system in solid phantoms

Eran A. Barnoy, Dror Fixler, Rachela Popovtzer, Tsviya Nayhoz, Krishanu Ray

Proceedings Volume 9721, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII; 972106 (2016) https://doi.org/10.1117/12.2207908
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Herein we describe promising results from the combination of fluorescent lifetime imaging microscopy (FLIM) and diffusion reflection (DR) medical imaging techniques. Three different geometries of gold nanoparticles (GNPs) were prepared: spheres of 20nm diameter, rods (GNRs) of aspect ratio (AR) 2.5, and GNRs of AR 3.3. Each GNP geometry was then conjugated using PEG linkers estimated to be 10nm in length to each of 3 different fluorescent dyes: Fluorescein, Rhodamine B, and Sulforhodamine B. DR provided deep-volume measurements (up to 1cm) from within solid, tissue-imitating phantoms, indicating GNR presence corresponding to the light used by recording light scattered from the GNPs with increasing distance to a photodetector. FLIM imaged solutions as well as phantom surfaces, recording both the fluorescence lifetimes as well as the fluorescence intensities. Fluorescence quenching was observed for Fluorescein, while metal-enhanced fluorescence (MEF) was observed in Rhodamine B and Sulforhodamine B – the dyes with an absorption peak at a slightly longer wavelength than the GNP plasmon resonance peak. Our system is highly sensitive due to the increased intensity provided by MEF, and also because of the inherent sensitivity of both FLIM and DR. Together, these two modalities and MEF can provide a lot of meaningful information for molecular and functional imaging of biological samples.

Conference Presentation

Citation Download Citation

Eran A. Barnoy, Dror Fixler, Rachela Popovtzer, Tsviya Nayhoz, and Krishanu Ray "Extremely sensitive dual imaging system in solid phantoms", Proc. SPIE 9721, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII, 972106 (22 April 2016); https://doi.org/10.1117/12.2207908

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