Plasmonic nanohole-based sub-diffraction-limited fluorescence microscopy for imaging of gliding biomolecules

Wonju Lee; Youngjin Oh; Kyujung Kim; Yoshiaki Kinosita; Nagisa Mikami; Takayuki Nishizaka; Donghyun Kim

doi:10.1117/12.2212903

22 April 2016 Plasmonic nanohole-based sub-diffraction-limited fluorescence microscopy for imaging of gliding biomolecules

Wonju Lee, Youngjin Oh, Kyujung Kim, Yoshiaki Kinosita, Nagisa Mikami, Takayuki Nishizaka, Donghyun Kim

Author Affiliations +

Proceedings Volume 9721, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII; 972107 (2016) https://doi.org/10.1117/12.2212903
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

In this presentation, we explore the feasibility of plasmonic nanohole-based sub-diffraction-limited nanoscopy for biomolecular imaging. The technique utilizes near-field distribution localized by surface plasmon localization on metallic nanoholes which is used to sample molecular fluorescence. The optimum geometry of nanohole arrays was determined by numerical analysis. The localization sampling was applied to reconstructing sub-diffraction-limited images of gliding microtubules with a 76 nm effective resolution in the lateral direction. Extraordinary light transmission was also employed to address enhancement of axial resolution using nanohole arrays, based on which extraction of gliding motions of bacteria was demonstrated with an axial resolution down to 50 nm.

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Wonju Lee, Youngjin Oh, Kyujung Kim, Yoshiaki Kinosita, Nagisa Mikami, Takayuki Nishizaka, and Donghyun Kim "Plasmonic nanohole-based sub-diffraction-limited fluorescence microscopy for imaging of gliding biomolecules", Proc. SPIE 9721, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII, 972107 (22 April 2016); https://doi.org/10.1117/12.2212903

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