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Proceedings Article

Monovalent plasmonic nanoparticles for biological applications

[+] Author Affiliations
Daeha Seo, Hyunjung Lee, Jung-uk Lee, Thomas J. Haas, Young-wook Jun

Univ. of California, San Francisco (United States)

Proc. SPIE 9722, Colloidal Nanoparticles for Biomedical Applications XI, 97220I (April 22, 2016); doi:10.1117/12.2208635
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From Conference Volume 9722

  • Colloidal Nanoparticles for Biomedical Applications XI
  • Wolfgang J. Parak; Marek Osinski; Xing-Jie Liang
  • San Francisco, California, United States | February 13, 2016

abstract

The multivalent nature of commercial nanoparticle imaging agents and the difficulties associated with producing monovalent nanoparticles challenge their use in biology, where clustering of target biomolecules can perturb dynamics of biomolecular targets. Here, we report production and purification of monovalent gold and silver nanoparticles for their single molecule imaging application. We first synthesized DNA-conjugated 20 nm and 40 nm gold and silver nanoparticles via conventional metal-thiol chemistry, yielding nanoparticles with mixed valency. By employing an anion-exchange high performance liquid chromatography (AE-HPLC) method, we purified monovalent nanoparticles from the mixtures. To allow efficient peak-separation resolution while keeping the excellent colloidal stability of nanoparticles against harsh purification condition (e.g. high NaCl), we optimized surface properties of nanoparticles by modulating surface functional groups. We characterized the monovalent character of the purified nanoparticles by hybridizing two complementary conjugates, forming dimers. Finally, we demonstrate the use of the monovalent plasmonic nanoprobes as single molecule imaging probes by tracking single TrkA receptors diffusing on the cell membrane and compare to monovalent quantum dot probes. © (2016) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Daeha Seo ; Hyunjung Lee ; Jung-uk Lee ; Thomas J. Haas and Young-wook Jun
" Monovalent plasmonic nanoparticles for biological applications ", Proc. SPIE 9722, Colloidal Nanoparticles for Biomedical Applications XI, 97220I (April 22, 2016); doi:10.1117/12.2208635; http://dx.doi.org/10.1117/12.2208635


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