Novel contrast mechanism for label free super-resolution imaging

Sergey A. Alexandrov; James McGrath; Hrebesh Subhash; Francesca Boccafoschi; Cinzia Giannini; Martin Leahy

doi:10.1117/12.2207458

9 March 2016 Novel contrast mechanism for label free super-resolution imaging

Sergey A. Alexandrov, James McGrath, Hrebesh Subhash, Francesca Boccafoschi, Cinzia Giannini, Martin Leahy

Proceedings Volume 9713, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIII; 971303 (2016) https://doi.org/10.1117/12.2207458
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

We present a novel super-resolution spectral encoding of spatial frequency (srSESF) approach to break the diffraction limit and dramatically improve resolution in lateral direction. The idea is to utilize additional information about the internal structure of the object to resolve features in the lateral direction. The novel contrast mechanism is realized by reconstruction and comparison of the axial spatial frequency (period) profiles at each image point to form super-resolution image. As a result, small features, unresolved by conventional microscopy, can be visualized. Numerical simulation and experiments confirm the super-resolution abilities of the srSESF approach without applying any labels.

Conference Presentation

Citation Download Citation

Sergey A. Alexandrov, James McGrath, Hrebesh Subhash, Francesca Boccafoschi, Cinzia Giannini, and Martin Leahy "Novel contrast mechanism for label free super-resolution imaging", Proc. SPIE 9713, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIII, 971303 (9 March 2016); https://doi.org/10.1117/12.2207458

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