Full Content is available to subscribers

Subscribe/Learn More  >
Proceedings Article

Label-free imaging of developing vasculature in zebrafish with phase variance optical coherence microscopy

[+] Author Affiliations
Yu Chen, Le A. Trinh, Scott E. Fraser

Univ. of Southern California (United States)

Jeff Fingler

Varocto, Inc. (United States)

Proc. SPIE 9716, Optical Methods in Developmental Biology IV, 97160A (March 9, 2016); doi:10.1117/12.2209653
Text Size: A A A
From Conference Volume 9716

  • Optical Methods in Developmental Biology IV
  • Andrew M. Rollins; Scott E. Fraser; Michael A. Choma
  • San Francisco, California, United States | February 13, 2016

abstract

A phase variance optical coherence microscope (pvOCM) has been created to visualize blood flow in the vasculature of zebrafish embryos, without using exogenous labels. The pvOCM imaging system has axial and lateral resolutions of 2 μm in tissue, and imaging depth of more than 100 μm. Imaging of 2–5 days post-fertilization zebrafish embryos identified the detailed structures of somites, spinal cord, gut and notochord based on intensity contrast. Visualization of the blood flow in the aorta, veins and intersegmental vessels was achieved with phase variance contrast. The pvOCM vasculature images were confirmed with corresponding fluorescence microscopy of a zebrafish transgene that labels the vasculature with green fluorescent protein. The pvOCM images also revealed functional information of the blood flow activities that is crucial for the study of vascular development. © (2016) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Yu Chen ; Jeff Fingler ; Le A. Trinh and Scott E. Fraser
" Label-free imaging of developing vasculature in zebrafish with phase variance optical coherence microscopy ", Proc. SPIE 9716, Optical Methods in Developmental Biology IV, 97160A (March 9, 2016); doi:10.1117/12.2209653; http://dx.doi.org/10.1117/12.2209653


Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).

Figures

Tables

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement


 

  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.