Forward versus backward polarization-resolved SHG imaging of collagen structure in tissues

Claire Teulon; Ivan Gusachenko; Gaël Latour; Marie-Claire Schanne-Klein

doi:10.1117/12.2208110

14 March 2016 Forward versus backward polarization-resolved SHG imaging of collagen structure in tissues

Claire Teulon, Ivan Gusachenko, Gaël Latour, Marie-Claire Schanne-Klein

Proceedings Volume 9712, Multiphoton Microscopy in the Biomedical Sciences XVI; 971218 (2016) https://doi.org/10.1117/12.2208110
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Second harmonic generation (SHG) is a powerful technique to observe fibrillar collagen without any staining and with a good contrast. More information about the molecular structure of collagen fibrils in tissues and their 3D distribution can be gained with polarization-resolved SHG imaging. Nevertheless, strong focusing is required for effective imaging and light propagation in tissues is complex and not thoroughly understood yet, preventing accurate and reproducible measurements. Theoretical analysis, vectorial numerical simulations and experiments were implemented to understand how the SHG signal builds up and how geometrical parameters affect polarization-resolved measurements in homogeneous collagen-rich tissues.

Conference Presentation

Citation Download Citation

Claire Teulon, Ivan Gusachenko, Gaël Latour, and Marie-Claire Schanne-Klein "Forward versus backward polarization-resolved SHG imaging of collagen structure in tissues", Proc. SPIE 9712, Multiphoton Microscopy in the Biomedical Sciences XVI, 971218 (14 March 2016); https://doi.org/10.1117/12.2208110

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