Nonlinear optical Stokes ellipsometric (NOSE) microscopy for imaging the nonlinear susceptibility tensors of collagen

Ximeng Y. Dow; Emma L. DeWalt; Shane Z. Sullivan; Paul D. Schmitt; Garth J. Simpson

doi:10.1117/12.2212944

14 March 2016 Nonlinear optical Stokes ellipsometric (NOSE) microscopy for imaging the nonlinear susceptibility tensors of collagen

Ximeng Y. Dow, Emma L. DeWalt, Shane Z. Sullivan, Paul D. Schmitt, Garth J. Simpson

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Proceedings Volume 9712, Multiphoton Microscopy in the Biomedical Sciences XVI; 97121S (2016) https://doi.org/10.1117/12.2212944
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Nonlinear optical Stokes ellipsometric (NOSE) microscopy was demonstrated for the analysis of collagen structure in a mouse tail section. NOSE is based on polarization-dependent second harmonic generation (SHG) imaging. The fast polarization-modulation was achieved using an electro-optic modulator (EOM), allowing for the potential of video-rate NOSE analysis. The signal to noise advantages associated with suppression of 1/f noise by rapid polarization modulation allowed reliable recovery of the local-frame tensor on a per-pixel basis. An iterative approach involving laboratory to local frame coordinate transformation was developed to recover the spatial distribution of local-frame nonlinear susceptibility tensor elements of collagen as well as the polar and azimuthal orientation angles of the collagen structure.

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Ximeng Y. Dow, Emma L. DeWalt, Shane Z. Sullivan, Paul D. Schmitt, and Garth J. Simpson "Nonlinear optical Stokes ellipsometric (NOSE) microscopy for imaging the nonlinear susceptibility tensors of collagen", Proc. SPIE 9712, Multiphoton Microscopy in the Biomedical Sciences XVI, 97121S (14 March 2016); https://doi.org/10.1117/12.2212944

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KEYWORDS

Collagen

Second-harmonic generation

Microscopy

Polarization

Tissues

Modulation

Chemical elements

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