Photoacoustic imaging of angiogenesis in subdermal islet transplant sites

Wei Shi; Rena Pawlick; Antonio Bruni; Yasmin Rafiei; Andrew R. Pepper; Boris Gala-Lopez; Min Choi; Andrew Malcolm; Roger J. Zemp; A. M. James Shapiro

doi:10.1117/12.2211247

18 March 2016 Photoacoustic imaging of angiogenesis in subdermal islet transplant sites

Wei Shi, Rena Pawlick, Antonio Bruni, Yasmin Rafiei, Andrew R. Pepper, Boris Gala-Lopez, Min Choi, Andrew Malcolm, Roger J. Zemp, A. M. James Shapiro

Author Affiliations +

Proceedings Volume 9708, Photons Plus Ultrasound: Imaging and Sensing 2016; 97080Q (2016) https://doi.org/10.1117/12.2211247
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Exogenous insulin administration is the mainstay treatment therapy for patients with Type-1 diabetes mellitus (T1DM). However, for select patients, clinical islet transplantation is an alternative therapeutic treatment. In this procedure, islets are transplanted into the hepatic portal vein, and despite improved success within the last decade, obstacles are still associated with this approach. It has been discovered that the subcutaneous space may be an effective alternative site for islet transplantation, and may provide advantages of easy access and potential for simple monitoring. The ability to monitor islet viability and the transplant microenvironment may be key to future success in islet transplantation. A subcutaneous device-less technique has been developed to facilitate angiogenesis in the islet transplant site, however, a method for monitoring the potential engraftment site have yet to be explored fully. Here we demonstrate the ability to track angiogenesis in mice with 1, 2, 3 and 4 weeks post-catheter implant on both sides of the abdomen using a FujiFilm VisualSonics Vevo-LAZR system. Quantitative analysis on vessel densities exhibited gradual vessel growth successfully induced by catheter implantation. Our study demonstrates the ability of employing photoacoustic and micro-ultrasound imaging to track angiogenesis around the catheter site prior to islet transplantation.

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Citation Download Citation

Wei Shi, Rena Pawlick, Antonio Bruni, Yasmin Rafiei, Andrew R. Pepper, Boris Gala-Lopez, Min Choi, Andrew Malcolm, Roger J. Zemp, and A. M. James Shapiro "Photoacoustic imaging of angiogenesis in subdermal islet transplant sites", Proc. SPIE 9708, Photons Plus Ultrasound: Imaging and Sensing 2016, 97080Q (18 March 2016); https://doi.org/10.1117/12.2211247

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