Development of automated high throughput single molecular microfluidic detection platform for signal transduction analysis

Po-Jung Huang; Sina Baghbani Kordmahale; Chao-Kai Chou; Hirohito Yamaguchi; Mien-Chie Hung; Jun Kameoka

doi:10.1117/12.2213259

21 March 2016 Development of automated high throughput single molecular microfluidic detection platform for signal transduction analysis

Po-Jung Huang, Sina Baghbani Kordmahale, Chao-Kai Chou, Hirohito Yamaguchi, Mien-Chie Hung, Jun Kameoka

Author Affiliations +

Proceedings Volume 9705, Microfluidics, BioMEMS, and Medical Microsystems XIV; 970511 (2016) https://doi.org/10.1117/12.2213259
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Signal transductions including multiple protein post-translational modifications (PTM), protein-protein interactions (PPI), and protein-nucleic acid interaction (PNI) play critical roles for cell proliferation and differentiation that are directly related to the cancer biology. Traditional methods, like mass spectrometry, immunoprecipitation, fluorescence resonance energy transfer, and fluorescence correlation spectroscopy require a large amount of sample and long processing time. “microchannel for multiple-parameter analysis of proteins in single-complex (mMAPS)”we proposed can reduce the process time and sample volume because this system is composed by microfluidic channels, fluorescence microscopy, and computerized data analysis. In this paper, we will present an automated mMAPS including integrated microfluidic device, automated stage and electrical relay for high-throughput clinical screening. Based on this result, we estimated that this automated detection system will be able to screen approximately 150 patient samples in a 24-hour period, providing a practical application to analyze tissue samples in a clinical setting.

Conference Presentation

Citation Download Citation

Po-Jung Huang, Sina Baghbani Kordmahale, Chao-Kai Chou, Hirohito Yamaguchi, Mien-Chie Hung, and Jun Kameoka "Development of automated high throughput single molecular microfluidic detection platform for signal transduction analysis", Proc. SPIE 9705, Microfluidics, BioMEMS, and Medical Microsystems XIV, 970511 (21 March 2016); https://doi.org/10.1117/12.2213259

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