Nano-enhanced optical delivery into targeted cells
(Conference Presentation)

Weldon Wright; Sanjay Pradhan

doi:10.1117/12.2218765

26 April 2016 Nano-enhanced optical delivery into targeted cells (Conference Presentation)

Weldon Wright, Sanjay Pradhan

Proceedings Volume 9690, Clinical and Translational Neurophotonics; Neural Imaging and Sensing; and Optogenetics and Optical Manipulation; 96902B (2016) https://doi.org/10.1117/12.2218765
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Nano-enhanced optical field of gold nanoparticles allowed the use of a continuous wave (cw) laser beam for efficient delivery of exogenous impermeable materials into targeted cells. Using this Nano-enhanced Optical Delivery (NOD) method, we show that large molecules could be delivered with low power cw laser with exposure time ~ 1sec. At such low power (and exposure), the non-targeted cells (not bound to gold nanoparticles) were not adversely affected by the laser beam. Further, by varying the size of the gold nanoparticles, cells could be exclusively sensitized to selective wavelengths of laser beam. In contrast other nanoparticles, gold nanoparticles were found to have lower cytotoxicity, making it better suited for clinical NOD. Further, as compared with pulsed lasers, cw (diode) lasers are compact, easy-to-use and therefore, NOD using cw laser beam has significant translational potential for delivery of impermeable bio-molecules to tissues in different organs. We will present optimization of NOD parameters for delivering different molecules to different cells. Success of this NOD method may lead to a new clinical approach for treating AMD and RP patients with geographic atrophy in retina.

Conference Presentation

Citation Download Citation

Weldon Wright and Sanjay Pradhan "Nano-enhanced optical delivery into targeted cells (Conference Presentation)", Proc. SPIE 9690, Clinical and Translational Neurophotonics; Neural Imaging and Sensing; and Optogenetics and Optical Manipulation, 96902B (26 April 2016); https://doi.org/10.1117/12.2218765

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KEYWORDS

Continuous wave operation

Nanoparticles

Gold

Molecules

Pulsed laser operation

Semiconductor lasers

Diodes

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