Quantification of neurotoxic effects on individual neuron cells using optical diffraction tomography
(Conference Presentation)

Jonghee Yoon; Su-a Yang; Kyoohyun Kim; YongKeun Park

doi:10.1117/12.2213780

27 April 2016 Quantification of neurotoxic effects on individual neuron cells using optical diffraction tomography (Conference Presentation)

Jonghee Yoon, Su-a Yang, Kyoohyun Kim, YongKeun Park

Author Affiliations +

Proceedings Volume 9718, Quantitative Phase Imaging II; 97180L (2016) https://doi.org/10.1117/12.2213780
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Parkinson’s disease (PD) is a common neurodegenerative disease that causes symptoms of postural instability and slowness of movement. Neurodegeneration in dopaminergic neurons at the substantia nigra has been reported as pathologic features, however, detailed mechanisms underlying neurodegeneration are still remain unclear. To investigate a neurodegenerative process, various imaging tools including phase contrast microscopy, electron microscopy, and fluorescence microscopy are utilized. However, these imaging methods provide qualitative information and require invasive approaches such as the use of fluorescence agents or chemical fixation procedures that disturb normal physiological conditions of neuron cells. In order to quantify the neurodegenerative process in a non-invasive manner, we exploited optical diffraction tomography (ODT). ODT is a 3D quantitative phase imaging method that measures 3D refractive index (RI) distributions of a sample which provide quantitative structural (volume, surface area, sphericity) and biochemical (protein concentration, total cellular dry mass) information. We investigated neurotoxic effects of MPP+ on SH-SY5Y cells by using quantitative information obtained from 3D RI distributions. We also performed temporal measurements of 3D RI distributions of an individual SH-SY5Y cell to analyze neurotoxic effects on intracellular vesicle dynamics.

Conference Presentation

Citation Download Citation

Jonghee Yoon, Su-a Yang, Kyoohyun Kim, and YongKeun Park "Quantification of neurotoxic effects on individual neuron cells using optical diffraction tomography (Conference Presentation)", Proc. SPIE 9718, Quantitative Phase Imaging II, 97180L (27 April 2016); https://doi.org/10.1117/12.2213780

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available