Quantitative phase imaging technologies to assess neuronal activity
(Conference Presentation)

Olivier Thouvenin; Mathias Fink; Claude Boccara

doi:10.1117/12.2211095

27 April 2016 Quantitative phase imaging technologies to assess neuronal activity (Conference Presentation)

Olivier Thouvenin, Mathias Fink, Claude Boccara

Proceedings Volume 9718, Quantitative Phase Imaging II; 97180S (2016) https://doi.org/10.1117/12.2211095
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Active neurons tends to have a different dynamical behavior compared to resting ones. Non-exhaustively, vesicular transport towards the synapses is increased, since axonal growth becomes slower. Previous studies also reported small phase variations occurring simultaneously with the action potential. Such changes exhibit times scales ranging from milliseconds to several seconds on spatial scales smaller than the optical diffraction limit. Therefore, QPI systems are of particular interest to measure neuronal activity without labels. Here, we report the development of two new QPI systems that should enable the detection of such activity. Both systems can acquire full field phase images with a sub nanometer sensitivity at a few hundreds of frames per second. The first setup is a synchronous combination of Full Field Optical Coherence Tomography (FF-OCT) and Fluorescence wide field imaging. The latter modality enables the measurement of neurons electrical activity using calcium indicators. In cultures, FF-OCT exhibits similar features to Digital Holographic Microscopy (DHM), except from complex computational reconstruction. However, FF-OCT is of particular interest in order to measure phase variations in tissues. The second setup is based on a Quantitative Differential Interference Contrast setup mounted in an epi-illumination configuration with a spectrally incoherent illumination. Such a common path interferometer exhibits a very good mechanical stability, and thus enables the measurement of phase images during hours. Additionally, such setup can not only measure a height change, but also an optical index change for both polarization. Hence, one can measure simultaneously a phase change and a birefringence change.

Conference Presentation

Citation Download Citation

Olivier Thouvenin, Mathias Fink, and Claude Boccara "Quantitative phase imaging technologies to assess neuronal activity (Conference Presentation)", Proc. SPIE 9718, Quantitative Phase Imaging II, 97180S (27 April 2016); https://doi.org/10.1117/12.2211095

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KEYWORDS

Phase measurement

Phase imaging

Digital holography

Neurons

Action potentials

Calcium

Diffraction

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