Hyperspectral imaging in SWIR: from stain-free microscopy to deep tissue imaging
(Conference Presentation)

Mikhail Y. Berezin

doi:10.1117/12.2216333

27 April 2016 Hyperspectral imaging in SWIR: from stain-free microscopy to deep tissue imaging (Conference Presentation)

Mikhail Y. Berezin

Author Affiliations +

Proceedings Volume 9723, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications VIII; 97230M (2016) https://doi.org/10.1117/12.2216333
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Recent advances in relatively unexplored short wave infrared (SWIR) range from 800-1600 nm detectors make wide-field imaging in this spectral range attractive to biology. The distinct advantages of SWIR region over the visible and near infrared (NIR) in tissue analysis are two-fold: (i) high abundance endogenous chromophores (i.e. water and lipids) enable tissue component differentiation based on wavelength-dependent absorption properties and (ii) the weak scattering of tissue permits better resolution of imaging in thick specimens. When combined with high spectral resolution, SWIR imaging produces a spectroscopic image, where every pixel corresponds to the entire high-resolution spectrum. This hyperspectral (HS) approach provides rich information about the relative abundance of individual chromophores and their interactions that contribute to the intensity and location of the optical signal. The presentation discusses the challenges in the SWIR-HS instrument design and data analysis and demonstrates some of the promising applications of this technology in life science and medicine.

Conference Presentation

Citation Download Citation

Mikhail Y. Berezin "Hyperspectral imaging in SWIR: from stain-free microscopy to deep tissue imaging (Conference Presentation)", Proc. SPIE 9723, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications VIII, 97230M (27 April 2016); https://doi.org/10.1117/12.2216333

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