Quantitative phase-digital holographic microscopy: a new imaging modality to identify original cellular biomarkers of diseases

P. Marquet; K. Rothenfusser; B. Rappaz; C. Depeursinge; P. Jourdain; P. J. Magistretti

doi:10.1117/12.2213454

2 May 2016 Quantitative phase-digital holographic microscopy: a new imaging modality to identify original cellular biomarkers of diseases

P. Marquet, K. Rothenfusser, B. Rappaz, C. Depeursinge, P. Jourdain, P. J. Magistretti

Author Affiliations +

Proceedings Volume 9718, Quantitative Phase Imaging II; 97180K (2016) https://doi.org/10.1117/12.2213454
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Quantitative phase microscopy (QPM) has recently emerged as a powerful label-free technique in the field of living cell imaging allowing to non-invasively measure with a nanometric axial sensitivity cell structure and dynamics. Since the phase retardation of a light wave when transmitted through the observed cells, namely the quantitative phase signal (QPS), is sensitive to both cellular thickness and intracellular refractive index related to the cellular content, its accurate analysis allows to derive various cell parameters and monitor specific cell processes, which are very likely to identify new cell biomarkers. Specifically, quantitative phase-digital holographic microscopy (QP-DHM), thanks to its numerical flexibility facilitating parallelization and automation processes, represents an appealing imaging modality to both identify original cellular biomarkers of diseases as well to explore the underlying pathophysiological processes.

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Citation Download Citation

P. Marquet, K. Rothenfusser, B. Rappaz, C. Depeursinge, P. Jourdain, and P. J. Magistretti "Quantitative phase-digital holographic microscopy: a new imaging modality to identify original cellular biomarkers of diseases", Proc. SPIE 9718, Quantitative Phase Imaging II, 97180K (2 May 2016); https://doi.org/10.1117/12.2213454

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