Estimation of protein dynamic states with single molecule fluorescence data analysis at microsecond scale

Kan Liu; Zhu Liu; Yi Peng; Yiming Ding; Chun Tang

doi:10.1117/12.2237125

14 September 2016 Estimation of protein dynamic states with single molecule fluorescence data analysis at microsecond scale

Kan Liu, Zhu Liu, Yi Peng, Yiming Ding, Chun Tang

Author Affiliations +

Proceedings Volume 9970, Optics and Photonics for Information Processing X; 99701D (2016) https://doi.org/10.1117/12.2237125
Event: SPIE Optical Engineering + Applications, 2016, San Diego, California, United States

Abstract

When timescale of the molecule kinetics faster than the bin time of millisecond order, the commercial single- molecule fluorescence resonance energy transfer (smFRET) data analysis tools may not clearly separate different states. We proposed a novel method to obtain the analysis result from smFRET raw data in microsecond scale. We introduced Akaike information criterion and non-stationarity measure in the fitting procedure to give an objective estimate about the number of states and populations. The results were used as initial values to make parameter estimation about equilibrium populations and transition rates from the photon trajectories based on an iterative optimizing procedure.

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Kan Liu, Zhu Liu, Yi Peng, Yiming Ding, and Chun Tang "Estimation of protein dynamic states with single molecule fluorescence data analysis at microsecond scale", Proc. SPIE 9970, Optics and Photonics for Information Processing X, 99701D (14 September 2016); https://doi.org/10.1117/12.2237125

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KEYWORDS

Proteins

Molecules

Data analysis

Molecular energy transfer

Resonance energy transfer

Fluorescence resonance energy transfer

Luminescence

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