Comparing natural and artificial carious lesions in human crowns by means of conventional hard x-ray micro-tomography and two-dimensional x-ray scattering with synchrotron radiation

Lea Maria Botta; Shane N. White; Hans Deyhle; Iwona Dziadowiec; Georg Schulz; Peter Thalmann; Bert Müller

doi:10.1117/12.2238487

4 October 2016 Comparing natural and artificial carious lesions in human crowns by means of conventional hard x-ray micro-tomography and two-dimensional x-ray scattering with synchrotron radiation

Lea Maria Botta, Shane N. White, Hans Deyhle, Iwona Dziadowiec, Georg Schulz, Peter Thalmann, Bert Müller

Author Affiliations +

Proceedings Volume 9967, Developments in X-Ray Tomography X; 99670S (2016) https://doi.org/10.1117/12.2238487
Event: SPIE Optical Engineering + Applications, 2016, San Diego, California, United States

Abstract

Dental caries, one of the most prevalent infectious bacterial diseases in the world, is caused by specific types of acid-producing bacteria. Caries is a disease continuum resulting from the earliest loss of ions from apatite crystals through gross cavitation. Enamel dissolution starts when the pH-value drops below 5.5. Neutralizing the pH-value in the oral cavity opposes the process of demineralization, and so caries lesions occur in a dynamic cyclic de-mineralizing/remineralizing environment. Unfortunately, biomimetic regeneration of cavitated enamel is not yet possible, although remineralization of small carious lesions occurs under optimal conditions. Therefore, the development of methods that can regenerate carious lesions, and subsequently recover and retain teeth, is highly desirable. For the present proceedings we analyzed one naturally occurring sub-surface and one artificially produced lesion. For the characterization of artificial and natural lesions micro computed tomography is the method of choice when looking to determine three-dimensional mineral distribution and to quantify the degree of mineralization. In this pilot study we elucidate that the de-mineralized enamel in natural and artificially induced lesions shows comparable X-ray attenuation behavior, thereby implying that the study protocol employed herein seems to be appropriate. Once we know that the lesions are comparable, a series of well-reproducible in vitro experiments on enamel regeneration could be performed. In order to quantify further lesion morphology, the anisotropy of the enamel’s nanostructure can be characterized by using spatially resolved, small-angle X-ray scattering. We wanted to demonstrate that the artificially induced defect fittingly resembles the natural carious lesion.

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Lea Maria Botta, Shane N. White, Hans Deyhle, Iwona Dziadowiec, Georg Schulz, Peter Thalmann, and Bert Müller "Comparing natural and artificial carious lesions in human crowns by means of conventional hard x-ray micro-tomography and two-dimensional x-ray scattering with synchrotron radiation", Proc. SPIE 9967, Developments in X-Ray Tomography X, 99670S (4 October 2016); https://doi.org/10.1117/12.2238487

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