Paper
19 October 2016 Research of the chemiluminescence detection apparatus for nutrients
Xiaoyi Xu, Yu Wang, Xuxiang Ni, Huimin Yan
Author Affiliations +
Proceedings Volume 10155, Optical Measurement Technology and Instrumentation; 1015520 (2016) https://doi.org/10.1117/12.2246772
Event: International Symposium on Optoelectronic Technology and Application 2016, 2016, Beijing, China
Abstract
The multifunctional nutrition analyzer, which integrates four detection functions, can make fast, accurate, quantitative analysis for a variety of nutrients. In this article we focus on researching the luminescence detection system. Compared with other means, luminescence detection needs no excitation light, and the detection sensitivity is improved due to the reduction of the background light. The apparatus consists of an displacement platform, a microporous plate, a combination of an aspheric lens and a plano-convex lens, an optical fiber and a photon counter connected with a computer. A theoretical light intensity formula is established as a reference and a comparison of the experimental data. In the experiment we applies ATP detection reagent as the experimental reagent, whose magnitudes of concentration are from 10-6 mol/L to 10-12 mol/L. The sensitivity of the apparatus could reach a magnitude of concentration of 0.1nmol/L, and it is estimated to be further improved by at least two magnitudes in theory with the system and the reagent optimized.
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Xiaoyi Xu, Yu Wang, Xuxiang Ni, and Huimin Yan "Research of the chemiluminescence detection apparatus for nutrients", Proc. SPIE 10155, Optical Measurement Technology and Instrumentation, 1015520 (19 October 2016); https://doi.org/10.1117/12.2246772
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KEYWORDS
Chemiluminescence

Luminescence

Photon counting

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