A microfluidic laser scattering sensor for label-free detection of waterborne pathogens

Huang Wei; Limei Yang; Feng Li

doi:10.1117/12.2246993

25 October 2016 A microfluidic laser scattering sensor for label-free detection of waterborne pathogens

Huang Wei, Limei Yang, Feng Li

Proceedings Volume 10156, Hyperspectral Remote Sensing Applications and Environmental Monitoring and Safety Testing Technology; 101561E (2016) https://doi.org/10.1117/12.2246993
Event: International Symposium on Optoelectronic Technology and Application 2016, 2016, Beijing, China

Abstract

A microfluidic-based multi-angle laser scattering (MALS) sensor capable of acquiring scattering pattern of single particle is demonstrated. The size and relative refractive index (RI) of polystyrene (PS) microspheres were deduced with accuracies of 60 nm and 0.001 by analyzing the scattering patterns. We measured scattering patterns of waterborne parasites i.e., cryptosporidium parvum (c.parvum) and giardia lamblia (g.lamblia), and some other representative species in 1 L water within 1 hour, and the waterborne parasites were identified with accuracy better than 96% by classification of distinctive scattering patterns with a support-vector-machine (SVM) algorithm. The system provides a promising tool for label-free and rapid detection of waterborne parasites.

Citation Download Citation

Huang Wei, Limei Yang, and Feng Li "A microfluidic laser scattering sensor for label-free detection of waterborne pathogens", Proc. SPIE 10156, Hyperspectral Remote Sensing Applications and Environmental Monitoring and Safety Testing Technology, 101561E (25 October 2016); https://doi.org/10.1117/12.2246993

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