All-optically integrated multimodality imaging system: combined photoacoustic microscopy, optical coherence tomography, and fluorescence imaging

Zhongjiang Chen; Sihua Yang; Da Xing

doi:10.1117/12.2245952

31 October 2016 All-optically integrated multimodality imaging system: combined photoacoustic microscopy, optical coherence tomography, and fluorescence imaging

Zhongjiang Chen, Sihua Yang, Da Xing

Author Affiliations +

Proceedings Volume 10024, Optics in Health Care and Biomedical Optics VII; 100240H (2016) https://doi.org/10.1117/12.2245952
Event: SPIE/COS Photonics Asia, 2016, Beijing, China

Abstract

We have developed a multimodality imaging system by optically integrating all-optical photoacoustic microscopy (AOPAM), optical coherence tomography (OCT) and fluorescence microscopy (FLM) to provide complementary information including optical absorption, optical back-scattering and fluorescence contrast of biological tissue. By sharing the same low-coherence Michelson interferometer, AOPAM and OCT could be organically optically combined to obtain the absorption and scattering information of the biological tissues. Also, owing to using the same laser source and objective lens, intrinsically registered photoacoustic and fluorescence signals are obtained to present the radiative and nonradiative transition process of absorption. Simultaneously photoacoustic angiography, tissue structure and fluorescence molecular in vivo images of mouse ear were acquired to demonstrate the capabilities of the optically integrated trimodality imaging system, which can present more information to study tumor angiogenesis, vasculature, anatomical structure and microenvironments in vivo.

Citation Download Citation

Zhongjiang Chen, Sihua Yang, and Da Xing "All-optically integrated multimodality imaging system: combined photoacoustic microscopy, optical coherence tomography, and fluorescence imaging", Proc. SPIE 10024, Optics in Health Care and Biomedical Optics VII, 100240H (31 October 2016); https://doi.org/10.1117/12.2245952

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