Rotary-scanning optical resolution photoacoustic microscopy

Weizhi Qi; Lei Xi

doi:10.1117/12.2245930

31 October 2016 Rotary-scanning optical resolution photoacoustic microscopy

Weizhi Qi, Lei Xi

Proceedings Volume 10024, Optics in Health Care and Biomedical Optics VII; 100242T (2016) https://doi.org/10.1117/12.2245930
Event: SPIE/COS Photonics Asia, 2016, Beijing, China

Abstract

Optical resolution photoacoustic microscopy (ORPAM) is currently one of the fastest evolving photoacoustic imaging modalities. It has a comparable spatial resolution to pure optical microscopic techniques such as epifluorescence microscopy, confocal microscopy, and two-photon microscopy, but also owns a deeper penetration depth. In this paper, we report a rotary-scanning (RS)-ORPAM that utilizes a galvanometer scanner integrated with objective to achieve rotary laser scanning. A 15 MHz cylindrically focused ultrasonic transducer is mounted onto a motorized rotation stage to follow optical scanning traces synchronously. To minimize the loss of signal to noise ratio, the acoustic focus is precisely adjusted to reach confocal with optical focus. Black tapes and carbon fibers are firstly imaged to evaluate the performance of the system, and then in vivo imaging of vasculature networks inside the ears and brains of mice is demonstrated using this system.

Citation Download Citation

Weizhi Qi and Lei Xi "Rotary-scanning optical resolution photoacoustic microscopy", Proc. SPIE 10024, Optics in Health Care and Biomedical Optics VII, 100242T (31 October 2016); https://doi.org/10.1117/12.2245930

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