Application study of transport intensity equation in quantitative phase reconstruction

Xiaojun Song; Wei Cheng; Chunjuan Wei; Liang Xue; Weijing Liu; Baodan Bai; Fenghong Chu

doi:10.1117/12.2246139

31 October 2016 Application study of transport intensity equation in quantitative phase reconstruction

Xiaojun Song, Wei Cheng, Chunjuan Wei, Liang Xue, Weijing Liu, Baodan Bai, Fenghong Chu

Proceedings Volume 10024, Optics in Health Care and Biomedical Optics VII; 100243F (2016) https://doi.org/10.1117/12.2246139
Event: SPIE/COS Photonics Asia, 2016, Beijing, China

Abstract

In order to improve detection speed and accuracy of biological cells, a quantitative non-interference optical phase recovery method is proposed in commercial microscope, taking the red blood cells as the classical phase objects. Three bright field micrographs were collected in the experiment. Utilizing the transport intensity equation (TIE), the quantitative phase distributions of red blood cell are gained and agree well with the previous optical phase models. Analysis shows that the resolution of introduced system reaches sub-micron. This method not only quickly gives quantitative phase distribution of cells, but also measures a large number of cells simultaneously. So it is potential in the use of real-time observing and quantitative analyzing of cells in vivo.

Citation Download Citation

Xiaojun Song, Wei Cheng, Chunjuan Wei, Liang Xue, Weijing Liu, Baodan Bai, and Fenghong Chu "Application study of transport intensity equation in quantitative phase reconstruction", Proc. SPIE 10024, Optics in Health Care and Biomedical Optics VII, 100243F (31 October 2016); https://doi.org/10.1117/12.2246139

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