Quantification of reactive oxygen species for photodynamic therapy

Zou Tan; Jinde Zhang; Lisheng Lin; Buhong Li

doi:10.1117/12.2246526

31 October 2016 Quantification of reactive oxygen species for photodynamic therapy

Zou Tan, Jinde Zhang, Lisheng Lin, Buhong Li

Proceedings Volume 10024, Optics in Health Care and Biomedical Optics VII; 100241C (2016) https://doi.org/10.1117/12.2246526
Event: SPIE/COS Photonics Asia, 2016, Beijing, China

Abstract

Photodynamic therapy (PDT) is an effective therapeutic modality that uses a light source to activate light-sensitive photosensitizers to treat both oncologic and nononcological indications. Photosensitizers are excited to the long-lived triplet state, and they react with biomolecules via type I or II mechanism resulted in cell death and tumor necrosis. Free radicals and radical ions are formed by electron transfer reactions (type I), which rapidly react with oxygen leading to the production of reactive oxygen species (ROS), including superoxide ions, hydroxyl radicals and hydrogen peroxide. Singlet molecular oxygen is produced in a Type II reaction, in which the excited singlet state of the photosensitizer generated upon photon absorption by the ground-state photosensitizer molecule undergoes intersystem crossing to a long-lived triplet state. In this talk, the fundmental mechanisms and detection techniques for ROS generation in PDT will be introduced. In particular, the quantification of singlet oxygen generation for pre-clinical application will be highlighted, which plays an essential role in the establishment of robust singlet oxygen-mediated PDT dosimetry.

Citation Download Citation

Zou Tan, Jinde Zhang, Lisheng Lin, and Buhong Li "Quantification of reactive oxygen species for photodynamic therapy", Proc. SPIE 10024, Optics in Health Care and Biomedical Optics VII, 100241C (31 October 2016); https://doi.org/10.1117/12.2246526

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available