DHMI: dynamic holographic microscopy interface

Xuefei He; Yujie Zheng; Woei Ming Lee

doi:10.1117/12.2242948

9 December 2016 DHMI: dynamic holographic microscopy interface

Xuefei He, Yujie Zheng, Woei Ming Lee

Proceedings Volume 10013, SPIE BioPhotonics Australasia; 1001332 (2016) https://doi.org/10.1117/12.2242948
Event: SPIE BioPhotonics Australasia, 2016, Adelaide, Australia

Abstract

Digital holographic microscopy (DHM) is a powerful in-vitro biological imaging tool. In this paper, we report a fully automated off-axis digital holographic microscopy system completed with a graphical user interface in the Matlab © environment. The interface primarily includes Fourier domain processing, phase reconstruction, aberration compensation and autofocusing. A variety of imaging operations such as region of interest selection, de-noising mode (filtering and averaging), low frame rate imaging for immediate reconstruction and high frame rate imaging routine (~ 27 fps) are implemented to facilitate ease of use.

Citation Download Citation

Xuefei He, Yujie Zheng, and Woei Ming Lee "DHMI: dynamic holographic microscopy interface", Proc. SPIE 10013, SPIE BioPhotonics Australasia, 1001332 (9 December 2016); https://doi.org/10.1117/12.2242948

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